Figure 7.

Fluorescence micrographs of the derived cells showing the distribution of the derived cells in the multi-tubular conduit with a length of 4.2 cm. The conduit was frozen sectioned along the direction perpendicular to the longitudinal axis at the (A) proximal, (B) center, and (C) distal positions, and along the direction parallel to the longitudinal axis at the (D) center and (E) boundary areas, respectively. S100 was stained with Alexa Fluor 488 anti-S100 (green), F-actins were stained with Alexa Fluor 555 phalloidin (red), and the cell nuclei were stained with DAPI (blue), respectively. The inserted images at the top right corners in (D) and (E) are the overlay of fluorescence micrograph and the corresponding bright-field image.