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. 2018 Nov 30;11:8589–8598. doi: 10.2147/OTT.S177019

Table S1.

Nested-PCR primers for the amplification of ABL1, BCR-ABL1 transcript identification and analysis of BCR-ABL1 mutational status

ABL1 – control Transcript identification Mutational status
Outer PCR For 5′-GGCCAGTAGCATCTGACTTTG (ABL1 exon 2)
Rev 5′-ATGGTACCAGGAGTGTTTCTCC (ABL1 exon 3)
For 5′-GACTGCAGCTCCAATGAGAAC (BCR exon 1)
Rev 5′-GTTTGGGCTTCACACCATTCC (ABL1 exon 3)
For 5′-CAGAACTCGCAACAGTCCTTC (BCR exon 1)
Rev 5′-CTTCGTCTGAGATACTGGATTCCT (ABL1 exon 9)
Inner PCR N/A For 5′-CAGAACTCGCAACAGTCCTTC (BCR exon 1)
Rev 5′-TTCCCCATTGTGATTATAGCCTA (ABL1 exon 3)
For 5′-GCAACAAGCCCACTGTCTAT (ABL1 exon 4)
Rev 5′-TGTTGTAGGCCAGGCTCTC (ABL1 exon 7)
For 5′-TGAGCAGGTTGATGACAGG (ABL1 exon 7)
Rev 5′-TGAGATACTGGATTCCTGGAAC (ABL1 exon 9)

Abbreviations: For, forward; N/A, not applicable; Rev, reverse.