Table 2. Primary antibodies used in immunohistochemistry.
Primary
Antibody |
Target | Dilution | Control | Cell Conditioning
Time |
||
---|---|---|---|---|---|---|
Positive Tissue | Negative
Tissue |
Negative
Antibody |
||||
TLR2 | Toll-like Receptor | 1:700 | Traumatic Oral
Ulcer |
Periapical
Scar |
Rabbit Isotype
IgG 1:500 |
Standard
(60 minutes) |
CD38 | Lymphocytes
Plasma Cell |
1:100 | Lingual Tonsil | Periapical
Scar |
Mouse Isotype
IgG 1:500 |
Standard
(60 minutes) |
CD68 | Macrophage
Monocyte |
1:200 | Mucocele | Periapical
Scar |
Mouse Isotype
IgG 1:500 |
Standard
(60 minutes) |
CD83 | Mature Dendritic
Cell |
1:20 | Lingual Tonsil | Periapical
Scar |
Mouse Isotype
IgG 1:500 |
Standard
(60 minutes) |