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. 2018 Nov 20;7:1819. [Version 1] doi: 10.12688/f1000research.16678.1

Table 3. Immunofluorescence protocols for primary antibodies.

Secondary
Labeling Antibody
Manufacturer Raised in React with Recommended
dilution range
Fluorescence Targeted primary antibody
(cells)
Alexa Fluor® 488 Invitrogen (A-11008)
Carisbad, CA, USA
Goat Rabbit 1:200 – 1:2000 Green TLR2
Alexa Fluor® 594 Invitrogen (A-21203)
Carisbad, CA, USA
Donkey Mouse 1:200 – 1:2000 Red CD38 (lymphocyte, plasma cell)
CD68 (macrophage)
Cd83 (matured dendritic cell)