Table 4. Exposure time for immunofluorescence imaging.
Secondary
Antibody |
Magnification | ||
---|---|---|---|
x200 | x400 | x1000 | |
Alexa Fluor 488® | 700 – 900 ms | 600 – 800ms | 300 – 500 ms |
Alexa Fluor 594® | 250 – 400 ms | 200 – 350ms | 150 – 250ms |
Secondary
Antibody |
Magnification | ||
---|---|---|---|
x200 | x400 | x1000 | |
Alexa Fluor 488® | 700 – 900 ms | 600 – 800ms | 300 – 500 ms |
Alexa Fluor 594® | 250 – 400 ms | 200 – 350ms | 150 – 250ms |