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. 2018 Nov 20;7:1819. [Version 1] doi: 10.12688/f1000research.16678.1

Table 4. Exposure time for immunofluorescence imaging.

Secondary
Antibody
Magnification
x200 x400 x1000
Alexa Fluor 488® 700 – 900 ms 600 – 800ms 300 – 500 ms
Alexa Fluor 594® 250 – 400 ms 200 – 350ms 150 – 250ms