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Pedigree of families. A, Patients P1 and P2 both carried a G>C transition at c.1965 NLRC4 encoding a tryptophan-to-cysteine substitution at p.W655. Solid symbols represent affected subjects, and open symbols represent unaffected subjects. Squares, Male subjects; circles, female subjects. B, Sequence alignment across species shows that this is a highly conserved locus.
