Fig 4.

p.W655 is located in the LRR domain of NLRC4. A, Schematic representation of NLRC4 domains, with the variant of interest shown in green. B and C, Ribbon representation of secondary structure of autoinhibited (PDB Code 4KXF²³; Fig 4, B) and active (PDB Code 6B5B²⁴; Fig 4, C) NLRC4. p.W655C NLRC4 is displayed in stick format in green. D and E, ASC speck quantification of WT and NLRC4 mutants by using flow cytometry (Fig 4, D), with NLRC4 expression in whole-cell lysate assessed by using Western blotting (Fig 4, E). Data are pooled from 5 independent experiments: *P < .05, **P < .01, ***P < .001, and ****P < .0001. NBD, Nucleotide binding domain; WHD, winged helix domain.