Fig 6.

Assessment of local conformational changes in response to NLRC4 activation. A, Ribbon representation of the region surrounding p.W655 displaying conformational changes between autoinhibited (dark purple) and active (light purple) NLRC4. p.W655 NLRC4 is highlighted in green. B, Residues potentially interacting with p.W655 at rest and maintaining NLRC4 in an autoinhibited conformation. C, Flow cytometric ASC speck quantification of WT and p.W655C NLRC4, along with mutations created to explore potential local interactions. Data were pooled from 3 independent experiments. ***P < .001 and ****P < .0001.