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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Biomaterials. 2018 Oct 20;189:48–59. doi: 10.1016/j.biomaterials.2018.10.022

Figure 6.

Figure 6.

LCP-GMP enhanced T-cell effector functions in tumors and lymphoid compartments. B16F10 tumor-bearing mice were given i.v. injections of LCP-GMP, free Gem and control LCP on days 8, 10, 12, 14 post tumor cell inoculation. On day 16, peripheral blood, tumors and spleens were collected and processed to single cell suspensions. Lymphocytes from (A-B) tumors, (C-D) peripheral blood and (E-F) spleens were polyclonally stimulated with PMA and ionomycin in vitro for 6 h, with the presence of brefeldin A in the last 4 h to block cytokine secretion. The productions of (A, C, E) IFN-γ and (B, D, F) TNF-α by CD8+ T cells in (A-B) tumors, (C-D) peripheral blood and (E-F) spleens were analyzed by intracellular cytokine staining. *p<0.05, ***p<0.0005, Untreated vs. LCP-GMP. (n=4 per group)