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. 2018 Dec 5;13(12):e0207621. doi: 10.1371/journal.pone.0207621

Fig 3. Functional disruption of PfPMV results in developmental arrest at the ring-to-trophozoite transition.

Fig 3

(A) Giemsa-stained thin blood films, showing the morphology of treated PMV-C5 parasites. Scale bar, 10 μm. The time-course of treatment and sampling of the cultures is indicated (top). (B) Growth assay showing relative replication rates following treatment of parental B11, PMV-C5 or PMV-F9 ring stage parasites with DMSO or RAP. Parasitaemia values (determined by FACS as described in Materials and Methods) are mean averages from 3 biological replicate experiments in different blood sources. The differences in growth rates between the RAP- and DMSO-treated PMV-C5 and PMV-F9 populations were extremely significant as determined by two-tailed t-test (t = 11.86, d.f. = 10, P<0.0001). Error bars, ±SD.