Table 4. Statistical analysis results for the multiplex gel-based and qPCR assays.
| Gel-based | ||||||||
| multiplex | ||||||||
| SS | SS 95% CI | SP | SP 95% CI | PPV | PPV 95% CI | NPV | NPV 95% CI | |
| porA | 70.4 | 62.4, 77.5 | 93.7 | 86.8, 97.6 | 94.7 | 88.8, 98.0 | 66.4 | 57.8, 74.3 |
| sodC | 80.9 | 73.7, 86.8 | 69.5 | 59.2, 78.5 | 80.9 | 73.8, 86.8 | 69.5 | 59.2, 78.5 |
| Cnl | 85.5 | 75.6, 92.5 | 98.7 | 92.9, 100 | 98.5 | 91.8, 100 | 87.2 | 78.3, 93.4 |
| A | 75.0 | 50.9, 91.3 | 99.2 | 95.9, 100 | 93.8 | 69.8, 99.8 | 96.3 | 91.6, 98.8 |
| W | 81.5 | 61.9, 93.7 | 98.4 | 94.3. 99.8 | 91.7 | 73.0, 99.0 | 96.1 | 91.1, 98.7 |
| X | 66.7 | 9.4, 99.1 | 98.0 | 94.2, 99.6 | 40.0 | 5.3, 85.3 | 99.3 | 96.3, 100 |
| B | 100.0 | 29.2, 100 | 99.3 | 96.3, 100 | 75.0 | 19.4, 99.4 | 100.0 | 97.5, 100 |
| C | 80.0 | 28.3, 99.5 | 100.0 | 97.5, 100 | 100.0 | 39.8, 100 | 99.3 | 96.3, 100 |
| Y | 80.0 | 44.4, 97.5 | 100.0 | 97.4, 100 | 100.0 | 63.1, 100 | 98.6 | 95.1, 99.8 |
| H | NA | NA | 100.0 | 97.6, 100 | NA | NA | 100.0 | 97.6, 100 |
| E | 100.0 | 2.5, 100 | 99.3 | 96.3, 100 | 50.0 | 1.3, 98.7 | 100.0 | 97.6, 100 |
| Z | NA | NA | 100.0 | 97.6, 100 | NA | NA | 100.0 | 97.6, 100 |
| porA/sodC | 88.8 | 82.7, 93.3 | 67.4 | 57.0, 76.6 | 81.3 | 74.6, 86.9 | 79.0 | 68.5, 87.3 |
| Real-time | ||||||||
| multiplex | ||||||||
| SS | SS 95% CI | SP | SP 95% CI | PPV | PPV 95% CI | NPV | NPV 95% CI | |
| porA | 96.1 | 91.6, 98.5 | 91.6 | 84.1, 96.3 | 94.8 | 90.0, 97.7 | 93.5 | 86.5, 97.6 |
| sodC | 94.7 | 89.9, 97.7 | 77.9 | 68.2, 85.8 | 87.3 | 81.2, 91.9 | 90.2 | 81.7, 95.7 |
| Cnl | 94.7 | 87.1, 98.5 | 100.0 | 95.3, 100 | 100.0 | 95.0, 100 | 95.0 | 87.7, 98.6 |
| A | 90.0 | 68.3, 98.8 | 100.0 | 97.2, 100 | 100.0 | 81.5, 100 | 98.5 | 94.7, 99.8 |
| W | 92.6 | 75.7, 99.1 | 100.0 | 97.1, 100 | 100.0 | 86.3, 100 | 98.4 | 94.4, 99.8 |
| X | 100.0 | 29.2, 100 | 99.3 | 96.3, 100 | 75.0 | 19.4, 99.4 | 100.0 | 97.5, 100 |
| B | 100.0 | 29.2, 100 | 100.0 | 97.5, 100 | 100.0 | 29.2, 100 | 100.0 | 97.6, 100 |
| C | 100.0 | 47.8, 100 | 100.0 | 97.5, 100 | 100.0 | 47.8, 100 | 100.0 | 97.5, 100 |
| Y | 90.0 | 55.5, 99.7 | 100.0 | 97.4, 100 | 100.0 | 66.4, 100 | 99.3 | 96.2, 100 |
| H | NA | NA | 100.0 | 97.6, 100 | NA | NA | 100.0 | 97.6, 100 |
| E | 100.0 | 2.5, 100 | 99.3 | 96.4, 100 | 50.0 | 1.3, 98.7 | 100.0 | 97.6, 100 |
| Z | NA | NA | 100.0 | 97.6, 100 | NA | NA | 100.0 | 97.6, 100 |
| porA/sodC | 96.1 | 91.6, 98.5 | 77.9 | 68.2, 85.8 | 87.4 | 81.4, 92.0 | 92.5 | 84.4, 97.2 |
Results of both PCR assays tested only in multiplex on a panel of 247 samples from Chad, Ethiopia, Ghana, Mali, Niger and Senegal. Sensitivity, Specificity, PPV and NPV were assessed for each primers pair and primers/probe in the multiplex assays and presented as percentages (%) with 95% confidence intervals (CI).