Table 3. Comparative testing of five methods for the detection of B. malayi PCR amplification products.

Sample #	Sample Content (# of A. aegypti mosquitoes / number of L3 larvae)	Real-Time PCR Result (Ct value)	Gel Result–Standard PCR	Test-Strip Result–Standard PCR	Gel Result -miniPCR	Test-Strip Result–miniPCR
22	5 / 1	32	+	+	+	+
32	5 / 1	27.7	+	+	+	+
38	5 / 1	30.2	+	+	+	+
47	5 / 1	30.5	+	+	+	+
2	10 / 1	29.9	+	+	+	+
17	10 / 1	29	+	+	+	+
29	10 / 1	30.6	+	+	+	+
37	10 / 1	29.7	+	+	+	+
49	10 / 1	32.2	+	+	+	+
9	15 / 1	28.9	+	+	+	+
11	15 / 1	26.9	+	+	+	+
18	15 / 1	32.9	+	+	+	+
23	15 / 1	29.4	+	+	+	+
1	20 / 1	26.8	+	+	+	+
4	20 / 1	28	+	+	+	+
6	20 / 1	34.8	+	+	+	+
25	20 / 1	32.4	+	+	+	+
43	20 / 1	36.5	+	+	+	+
45	20 / 1	28.6	+	+	+	+
46*	20 / 1	39.8	-	+	-	+
13*	25 / 1	-	-	+	-	-
21	25 / 1	36.5	+	+	+	+
30	25 / 1	28.6	+	+	+	+
33	25 / 1	27.5	+	+	+	+
35	25 / 1	31.4	+	+	+	+
40	25 / 1	30.5	+	+	+	+
41	25 / 1	27.5	+	+	+	+
44	25 / 1	29.9	+	+	+	+
3	5 / 0	-	-	-	-	-
5	5 / 0	-	-	-	-	-
7	5 / 0	-	-	-	-	-
8	5 / 0	-	-	-	-	-
10	5 / 0	-	-	-	-	-
42	5 / 0	-	-	-	-	-
12	10 / 0	-	-	-	-	-
19	10 / 0	-	-	-	-	-
20	10 / 0	-	-	-	-	-
28	10 / 0	-	-	-	-	-
50	10 / 0	-	-	-	-	-
14	15 / 0	-	-	-	-	-
15	15 / 0	-	-	-	-	-
24	15 / 0	-	-	-	-	-
27	15 / 0	-	-	-	-	-
39	15 / 0	-	-	-	-	-
48	15 / 0	-	-	-	-	-
26	20 / 0	-	-	-	-	-
34	20 / 0	-	-	-	-	-
36	20 / 0	-	-	-	-	-
16	25 / 0	-	-	-	-	-
31	25 / 0	-	-	-	-	-

* Testing of sample produced a false negative result by one or more detection methods.