Skip to main content
. 2018 Nov 21;14(11):e1007814. doi: 10.1371/journal.pgen.1007814

Fig 5. IMP1 mediates endocytic and autophagic vesicle dynamics.

Fig 5

(A) FM4-64 staining shows endocytic vesicles targeting vacuoles in Δimp1 and WT. After 5h, FM4-64 is not distributed throughout the full vesicular network in Δimp1 mycelia. Scale bar = 2 μm. (B) Monodansylcadaverine (MDC) staining revealed both reduced numbers of autophagic vacuoles in Δimp1 compared to WT, and reduced overlap with FM4-64 stained compartments. Mycelia from WT and Δimp1 were incubated in GMM for 16 h then stained with 1 μg per ml FM4-64 and 40 μM MDC for 5 h in water. Scale bar = 10 μm. (C) IMP1 is required for endosomal and autophagosomal membrane vesicle trafficking to acidified autophagic vacuoles, and might also be required for supplying endosomal membranes for phagophore expansion.