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. 2018 Nov 21;14(11):e1007814. doi: 10.1371/journal.pgen.1007814

Fig 10. V-ATPase function is not required for biotrophic interfacial membrane integrity.

Fig 10

Leaf sheaths infected with the indicated strains were treated with (A) 10μM concanamycin A (ConA) or (B) 1 μM bafilomycin A1 (BafA1) at 36 hpi and viewed at 44 hpi. (A,B) Arrows indicate appressoria on the leaf surface. Scale bars = 10 μm. NT = no treatment. Proportion of infected rice cells represented by these images are shown in S1 Table. (C) BafA1 and ConA treatments suggest V-ATPase and vacuole function is required for cell-to-cell movement but not for maintaining biotrophic interfaces, which therefore must be dependent on an earlier stage of autophagy.