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. 2018 Dec 5;13(12):e0207296. doi: 10.1371/journal.pone.0207296

Table 2. Primers used to amplify fragments of cDNA.

Gene Exons Primers Sequence 5'-> 3' Size
(bp)
NEB
NM_001271208.1
20–28 NEB_20–28_EXT-D GCATTAATGACGATCCCAAGATG 929
NEB_20–28_EXT-R ACTTGGAGCATATCAAGAGGTGC
NEB_20–28_INT-D GAACTTCAGTGAGGCTAGATATAAAGACT 550
NEB_20–28_INT-R CTTGTACATCACATCGCTGGTGTT
73–77 NEB_73–77_EXT-D TACAGCAGCCCAGTGGACATGCTTG 682
NEB_73–77_EXT-R GGATCTTGGCCACATGGATGGACCAC
NEB_73–77_INT-D TAGTGATACTATCTACCGTCAGCGT 261
NEB_73–77_INT-R GACTCTTCCAAAGCAAGTTTATAGA
111–114 NEB_111–114_INT-D TCAACTTTACGCCTGTGGATGACAG 396
NEB_111–114_INT-R TCTCATTCTGAATCTGGCCTGCATGC
NEB_111–114_EXT-D ACTGCAGAGCGATAATGTTTACAAG 751
NEB_111–114_EXT-R TCTGTAGACATTATCACTCTGTAGGTC

EXT: primers used for amplification; INT: primers used for sequencing.