Table 2. Primers used to amplify fragments of cDNA.
Gene | Exons | Primers | Sequence 5'-> 3' |
Size (bp) |
---|---|---|---|---|
NEB NM_001271208.1 |
20–28 | NEB_20–28_EXT-D | GCATTAATGACGATCCCAAGATG | 929 |
NEB_20–28_EXT-R | ACTTGGAGCATATCAAGAGGTGC | |||
NEB_20–28_INT-D | GAACTTCAGTGAGGCTAGATATAAAGACT | 550 | ||
NEB_20–28_INT-R | CTTGTACATCACATCGCTGGTGTT | |||
73–77 | NEB_73–77_EXT-D | TACAGCAGCCCAGTGGACATGCTTG | 682 | |
NEB_73–77_EXT-R | GGATCTTGGCCACATGGATGGACCAC | |||
NEB_73–77_INT-D | TAGTGATACTATCTACCGTCAGCGT | 261 | ||
NEB_73–77_INT-R | GACTCTTCCAAAGCAAGTTTATAGA | |||
111–114 | NEB_111–114_INT-D | TCAACTTTACGCCTGTGGATGACAG | 396 | |
NEB_111–114_INT-R | TCTCATTCTGAATCTGGCCTGCATGC | |||
NEB_111–114_EXT-D | ACTGCAGAGCGATAATGTTTACAAG | 751 | ||
NEB_111–114_EXT-R | TCTGTAGACATTATCACTCTGTAGGTC |
EXT: primers used for amplification; INT: primers used for sequencing.