Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-Myc (mouse monoclonal) |
Roche | 11667149001; RRID: AB_390912 |
(1:2000) |
| Antibody | anti-HA (12CA5) (mouse monoclonal) |
Roche | 11583816001; RRID: AB_514506 |
(1:5000) |
| Antibody | anti-ACTIN (rabbit polyclonal) |
Sigma-Aldrich | A2066; RRID:AB_476693 |
(1:2000) |
| Antibody | anti-Hsp90 (rabbit polyclonal) |
PMID: 23434373 | (1:10000) | |
| Antibody | anti-GST (rabbit polyclonal) |
GeneTex | GTX110736; RRID: AB_1949427 |
(1:2000) |
| Antibody | anti-Pgk1 (mouse monoclonal) |
Invitrogen | 459250 | (1:5000) |
| Antibody | anti-(P) S148-Ids2 (rabbit purified polyclonal antibody) |
This paper | (1:1000) | |
| Antibody | anti-Firefly Luciferase |
GeneTex | GTX125849; RRID: AB_11173184 |
(1:5000) |
| Commercial assay or kit |
EnzChek phosphate assay kit |
Thermo Fisher Scientific |
E6646 | |
| Commercial assay or kit |
KAPA SYBR FAST qPCR Master Mix Kit |
Sigma- Aldrich |
KK4600 | |
| Other | Calmodulin Sepharose 4B |
GE Healthcare |
17-0529-01 | Affinity resin |
| Other | TALON Superflow |
GE Healthcare |
28-9574-99 | Affinity resin |
| Other | IgG Sepharose 6 Fast Flow |
Amersham Pharmacia Biotech AB |
17-0969-01 | Affinity resin |
| Other | Glutathione Sepharose 4 Fast Flow |
GE Healthcare |
17-5132-01 | Affinity resin |
| Chemical compound, drug |
Deoxycorticosterone acetate |
Sigma- Aldrich |
D7000 | |
| Chemical compound, drug |
Protease inhibitor cocktail tablets |
Roche | 4693132001 | |
| Chemical compound, drug |
PKA, catalytic subunit, bovine heart |
Millipore | 539576 | |
| Software | Image J | RRID: SCR_003070 | Image analysis |
|
| Software | GraphPad Prism5 |
RRID: SCR_002798 | Statistical analysis and graph representation |