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. 2018 Nov 21;7:e39756. doi: 10.7554/eLife.39756

Key resources table.

Reagent type
(species) or resource
Designation Source or reference Identifiers Additional
information
Bacterial strain E. coli: OP50 Caenorhabditis
Genetics Center
RRID:
WB-STRAIN:OP50
Genetic reagent
(C. elegans)
Strain wild type N2 Caenorhabditis
Genetics Center
RRID:WB-STRAIN:N2_
(ancestral)
Genetic reagent
(C. elegans)
CZ10969: Pmec-7-GFP(muIs32) II Considered as‘WT’ in many axotomy experiments
Genetic reagent
(C. elegans)
CZ10175: Pmec-4-GFP(zdIs5) I Considered as ‘WT’ in many axotomy experiments
Genetic reagent
(C. elegans)
CZ25411: nmat-2(ju1512) I/hT2 I, III;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ25415: nmat-2(ju1514) I/hT2 I, III; 
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ17633: nmat-2(tm2905) I/hT2 I, III; 
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ24324: Pmec-7-GFP(muIs32) II; qns-1(ju1563) IV/mIs11 sd IV
Genetic reagent
(C. elegans)
CZ25642: Pmec-4-GFP(zdIs5) I; 
Pmec-7-GFP(muIs32) II
Genetic reagent
(C.elegans)
CZ25534: nmrk-1(ok2571) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ24241: Pmec-4-GFP(zdIs5) I; 
nprt-1(tm6342) IV
Genetic reagent
(C. elegans)
CZ24242: Pmec-4-GFP(zdIs5) I; 
pnc-1(tm3502) IV
Genetic reagent
(C. elegans)
CZ24802: Pmec-4-GFP(zdIs5) I;
pnc-2(tm6438) IV
Genetic reagent
(C. elegans)
CZ25466: Pmec-7-GFP(muIs32) II;
juSi347[nmat-2 gDNA] IV
Genetic reagent
(C. elegans)
CZ25469: nmat-2(tm2905) I;Pmec-7-GFP(muIs32) II;
juSi347[nmat-2 gDNA] IV
Genetic reagent
(C. elegans)
CZ26216: Pmec-7-GFP(muIs32) II;
Ex[Pmec-4-nmat-2(juEx7834)]
Genetic reagent
(C. elegans)
CZ26217: Pmec-7-GFP(muIs32) II;
Ex[Pmec-4-nmat-2(juEx7835)]
Genetic reagent
(C. elegans)
CZ26220: Pmec-7-GFP(muIs32) II;
Ex[Pcol-12-nmat-2(juEx7838)]
Genetic reagent
(C. elegans)
CZ26221: Pmec-7-GFP(muIs32) II;
Ex[Pcol-12-nmat-2(juEx7839)]
Genetic reagent
(C. elegans)
CZ26218: Pmec-7-GFP(muIs32) II;
Ex[Pmtl-2-nmat-2(juEx7836)]
Genetic reagent
(C. elegans)
CZ26219: Pmec-7-GFP(muIs32) II;
Ex[Pmtl-2-nmat-2(juEx7837)]
Genetic reagent
(C. elegans)
CZ26222: nmat-2(ju1512) I/hT2 I, III;
Pmec-7-GFP(muIs32) II;
Ex[Pmec-4-nmat-2(juEx7840)]
Genetic reagent
(C. elegans)
CZ26223: nmat-2(ju1512) I/hT2 I, III;
Pmec-7-GFP(muIs32) II;
Ex[Pmec-4-nmat-2(juEx7841)]
Genetic reagent
(C. elegans)
CZ26224: nmat-2(ju1512) I/hT2 I, III;
Pmec-7-GFP(muIs32) II;
Ex[Pcol-12-nmat-2(juEx7842)]
Genetic reagent
(C. elegans)
CZ26225: nmat-2(ju1512) I/hT2 I, III;
Pmec-7-GFP(muIs32) II;
Ex[Pcol-12-nmat-2(juEx7843)]
Genetic reagent
(C. elegans)
CZ26310: nmat-2(ju1512) I/hT2 I, III;
Pmec-7-GFP(muIs32) II;
Ex[Pmtl-2-nmat-2(juEx7836)]
Genetic reagent
(C. elegans)
CZ26311: nmat-2(ju1512) I/hT2 I, III;
Pmec-7-GFP(muIs32) II;
Ex[Pmtl-2-nmat-2(juEx7837)]
Genetic reagent
(C. elegans)
CZ26332: nmat-2(ju1512) I/hT2 I, III;
Pmec-7-GFP(muIs32) II;
Ex[Pmtl-2, col-12::nmat-2(juEx7853)]
Genetic reagent
(C. elegans)
CZ26333: nmat-2(ju1512) I/hT2 I, III;
Pmec-7-GFP(muIs32) II;
Ex[Pmtl-2, col-12::nmat-2(juEx7854)]
Genetic reagent
(C. elegans)
CZ26285: nmat-2(ju1512) I/hT2 I, III;
Pmec-7-GFP(muIs32) II;
Ex[Pmtl-2, col-12, mec-4::nmat-2(juEx7850)]
Genetic reagent
(C. elegans)
CZ26286: nmat-2(ju1512) I / hT2 I, III;
Pmec-7-GFP(muIs32) II;
Ex[Pmtl-2, col-12, mec-4::nmat-2(juEx7851)]
Genetic reagent
(C. elegans)
CZ26391: jph-1(ok2823) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ22032: Pmec-4-GFP(zdIs5) I;
anoh-1(tm4762) III
Genetic reagent
(C. elegans)
CZ22033: Pmec-4-GFP(zdIs5) I; 
anoh-2(tm4796) IV
Genetic reagent
(C. elegans)
CZ26325: Pmec-4-GFP(zdIs5) I;
anoh-1(tm4762) III;
anoh-2(tm4796) IV
Genetic reagent
(C. elegans)
CZ26069: Pmec-4-GFP(zdIs5) I;
obr-1(xh16) III
Genetic reagent
(C. elegans)
CZ24555: Pmec-4-GFP(zdIs5) I; 
obr-2 (xh17) V
Genetic reagent
(C. elegans)
CZ24556: Pmec-4-GFP(zdIs5) I;
obr-3(tm1087) X
Genetic reagent
(C. elegans)
CZ24557: obr-4(tm1567) I;
Pmec-4-GFP(zdIs5) I
Genetic reagent
(C. elegans)
CZ25696: obr-4(tm1567) I;
Pmec-4-GFP(zdIs5) I;
obr-1(xh16) III; obr-2(xh17) V; 
obr-3(tm1087) X
Genetic reagent
(C. elegans)
CZ26375: Pmec-4-GFP(zdIs5) I; 
esyt-2(ju1409) III
Genetic reagent
(C. elegans)
CZ26570: juIs540 [Pmec-4-mKate2-ESYT-2]; 
juEx7807[Pmec-4-GFP-PISY-1]
Genetic reagent
(C. elegans)
CZ24897: juEx7604 [Pmec-4-GFP-ESYT-2]
Genetic reagent
(C. elegans)
CZ22087: Pmec-7-GFP(muIs32) II;
atgl-1(tm3116) III / hT2 I, III
Genetic reagent
(C. elegans)
CZ22536: Pmec-7-GFP(muIs32) II;
hosl-1(gk278589) X
Genetic reagent
(C. elegans)
CZ22006: Pmec-7-GFP(muIs32) II;
abhd-5.1(ok3722) V
Genetic reagent
(C. elegans)
CZ21968: Pmec-7-GFP(muIs32) II;
abhd-5.2(ok3245) V
Genetic reagent
(C. elegans)
CZ22007: lid-1(gk575511) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ22163: Pmec-7-GFP(muIs32) II;
abhd-5.2(ok3245) V abhd-5.1(ju1282) V
Genetic reagent
(C. elegans)
CZ22166: lid-1(gk575511) I;
Pmec-7-GFP(muIs32) II;
abhd-5.2(ok3245) abhd-5.1(ju1282) V
Genetic reagent
(C. elegans)
CZ22686: Pmec-7-GFP(muIs32) II;
lipl-1(tm1954) V
Genetic reagent
(C. elegans)
CZ22688: Pmec-7-GFP(muIs32) II;
lipl-3(tm4498) V
Genetic reagent
(C. elegans)
CZ22535: Pmec-7-GFP(muIs32) II;
lipl-4(tm4417) V
Genetic reagent
(C. elegans)
CZ24364: Pmec-4-GFP(zdIs5) I;
lgg-1(tm3489) II/ mIn1 II
Genetic reagent
(C. elegans)
CZ23325: Pmec-7-GFP(muIs32) II;
lgg-2(tm6474) IV
Genetic reagent
(C. elegans)
CZ23322: Pmec-7-GFP(muIs32) II;
hlh-30(tm1978) IV
Genetic reagent
(C. elegans)
CZ14408: Pmec-7-GFP(muIs32) II;
mxl-3(ok1947) X
Genetic reagent
(C. elegans)
CZ22541: nhr-49(nr2041) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ22510: Pmec-7-GFP(muIs32) II;
nhr-80(tm1011) III
Genetic reagent
(C. elegans)
CZ25587: Pmec-4-GFP(zdIs5) I;
cka-1(tm1241) IV
Genetic reagent
(C. elegans)
CZ25549: Pmec-4-GFP(zdIs5) I; 
cka-2(tm841) X
Genetic reagent
(C. elegans)
CZ25403: Pmec-4-GFP(zdIs5) I; 
cka-1(tm1241) IV; cka-2(tm841) X.
Genetic reagent
(C. elegans)
CZ25370: Pmec-4-GFP(zdIs5) I;
pcyt-1(et9) X
Genetic reagent
(C. elegans)
CZ25790: Pmec-4-GFP(zdIs5) I;
pcyt-2.1(gk440213) I
Genetic reagent
(C. elegans)
CZ25368: Pmec-4-GFP(zdIs5) I; 
pcyt-2.2(ok2179) X
Genetic reagent
(C. elegans)
CZ25992: Pmec-4-GFP(zdIs5) I; pcyt-2.1(gk440213) I;
pcyt-2.2(ok2179) X
Genetic reagent
(C. elegans)
CZ26521: Pmec-4-GFP(zdIs5) I; pcyt-2.1(gk440213) I;
pcyt-1(et9) X 
Genetic reagent
(C. elegans)
CZ25369: Pmec-4-GFP(zdIs5) I; 
cept-1(et10) X
Genetic reagent
(C. elegans)
CZ26423: Pmec-4-GFP(zdIs5) I; 
cept-2(ok3135) V
Genetic reagent
(C. elegans)
CZ19835: Pmec-4-GFP(zdIs5) I; 
kel-8(tm5214) V
Genetic reagent
(C. elegans)
CZ23911: kel-20(tm3676) I;
mec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ18224: Pmec-4-GFP(zdIs5) I;
ivns-1(ok3171) X
Genetic reagent
(C. elegans)
CZ18225: Pmec-4-GFP(zdIs5) I;
ivns-1(gk252) X
Genetic reagent
(C. elegans)
CZ25508: Pmec-4-GFP(zdIs5) I;
ivns-1(ok3171) X; 
Ex[ivns-1_gDNA(juEx7673)]
Genetic reagent
(C. elegans)
CZ25509: Pmec-4-GFP(zdIs5) I;
ivns-1(ok3171) X;
Ex[ivns-1_gDNA(juEx7674)]
Genetic reagent
(C. elegans)
CZ25510: Pmec-4-GFP(zdIs5) I;
ivns-1(gk252) X;
Ex[ivns-1_gDNA(juEx7673)]
Genetic reagent
(C. elegans)
CZ25511: Pmec-4-GFP(zdIs5) I;
ivns-1(gk252) X;
Ex[ivns-1_gDNA(juEx7674)]
Genetic reagent
(C. elegans)
CZ24755: juEx7584[Pesyt-2-GFP]
Genetic reagent
(C. elegans)
CZ21465: Pmec-4-GFP(zdIs5) I;
epi-1(gm121) IV
Genetic reagent
(C. elegans)
CZ21463: Pmec-7-GFP(muIs32) II;
emb-9(tk75) III
Genetic reagent
(C. elegans)
CZ21198: Pmec-7-GFP(muIs32) II;
adt-1(cn30) X
Genetic reagent
(C. elegans)
CZ20937: Pmec-4-GFP(zdIs5) I;
adt-2(wk156) X
Genetic reagent
(C. elegans)
CZ21004: Pmec-4-GFP(zdIs5) I;
adt-3 (T19D2.1) (ok923) X
Genetic reagent
(C. elegans)
CZ26611: Pmec-7-GFP(muIs32) II;
gon-1(e1254) IV / +
Genetic reagent
(C. elegans)
CZ23908: rab-8(tm2526) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ23909: rab-11.2(tm2081) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ20682: Pmec-7-GFP(muIs32) II;
nex-1(gk148) III
Genetic reagent
(C. elegans)
CZ20683: Pmec-7-GFP(muIs32) II;
nex-2(ok764) III
Genetic reagent
(C. elegans)
CZ20684: Pmec-7-GFP(muIs32) II;
nex-3(gk385) III
Genetic reagent
(C. elegans)
CZ20685: Pmec-7-GFP(muIs32) II;
nex-4(gk102) V
Genetic reagent
(C. elegans)
CZ14006: Pmec-7-GFP(muIs32) II;
mec-17(ok2109) IV
Genetic reagent
(C. elegans)
CZ14008: Pmec-7-GFP(muIs32) II;
atat-2(ok2415) X
Genetic reagent
(C. elegans)
CZ14848: mec-17(ok2109) IV; atat-2(ok2415) X;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ17720: Pmec-7-GFP(muIs32) II;
mec-12(tm5083) III
Genetic reagent
(C. elegans)
CZ9247: tba-1(ok1135) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ26688: Pmec-4-GFP(zdIs5) I;
tba-7(gk787939) III
Genetic reagent
(C. elegans)
CZ26833: Pmec-4-GFP(zdIs5) I;
tba-7(u1015) III
Genetic reagent
(C. elegans)
CZ26635: Pmec-4-GFP(zdIs5) I;
mec-7(ok2152) X
Genetic reagent
(C. elegans)
CZ10615: Pmec-4-GFP(zdIs5) I;
tbb-2(gk129) III
Genetic reagent
(C. elegans)
CZ11083: Pmec-4-GFP(zdIs5) I;
tbb-4(ok1461) X
Genetic reagent
(C. elegans)
CZ10810: Pmec-4-GFP(zdIs5) I;
tbb-6(tm2004) V
Genetic reagent
(C. elegans)
CZ21461: Pmec-4-GFP(zdIs5) I;
fli-1(ky535) III
Genetic reagent
(C. elegans)
CZ21199: Pmec-7-GFP(muIs32) II;
gsnl-1(ok2979) V
Genetic reagent
(C. elegans)
CZ10888: viln-1(ok2413) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ13606: Pmec-4-GFP(zdIs5) I;
twf-2(ok3564) X
Genetic reagent
(C. elegans)
CZ20063: Pmec-4-GFP(zdIs5) I;
mfsd-6(ju833) III
Genetic reagent
(C. elegans)
CZ19827: Pmec-4-GFP(zdIs5) I;
mfsd-6(tm3356) III
Genetic reagent
(C. elegans)
CZ24417: Pmec-4-GFP(zdIs5) I;
mfsd-6(tm3356) III;
Prgef-1-mfsd-6(juEx6079)
Genetic reagent
(C. elegans)
CZ21030: Pmec-4-GFP(zdIs5) I;
C05D9.7(ok2931) X
Genetic reagent
(C. elegans)
CZ25317: Pmec-4-GFP(zdIs5) I;dpy-10(e128) II
Genetic reagent
(C. elegans)
CZ23667: Pmec-4-GFP(zdIs5) I;F35G2.1(ok1669) IV
Genetic reagent
(C. elegans)
CZ23772: Pmec-4-GFP(zdIs5) I;gly-2(tm839) I
Genetic reagent
(C. elegans)
CZ17890: Pmec-4-GFP(zdIs5) I;osm-11(rt142) X
Genetic reagent
(C. elegans)
CZ17021: Pmec-4-GFP(zdIs5) I;zig-1(ok784) II
Genetic reagent
(C. elegans)
CZ17023: Pmec-4-GFP(zdIs5) I;zig-3(gk33) X
Genetic reagent
(C. elegans)
CZ17024: Pmec-4-GFP(zdIs5) I;zig-3(ok1476) X
Genetic reagent
(C. elegans)
CZ22031: abts-1(ok1566) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ21461: Pmec-4-GFP(zdIs5) I; 
fli-1(ky535) III
Genetic reagent
(C. elegans)
CZ17435: Pmec-7-GFP(muIs32) II;mec-12(e1605) III
Genetic reagent
(C. elegans)
CZ17637: Pmec-4-GFP(zdIs5) I;tba-9(ok1858) X
Genetic reagent
(C. elegans)
CZ20033: vab-10(e698) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ17099: Pmec-4-GFP(zdIs5) I;
plk-1(or683ts) III
Genetic reagent
(C. elegans)
CZ17285: Pmec-4-GFP(zdIs5) I;
svh-2(tm737) X
Genetic reagent
(C. elegans)
CZ19343: Pmec-4-GFP(zdIs5) I;
cpr-1(ok1344) V
Genetic reagent
(C. elegans)
CZ19200:dhhc-11(gk1105) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ22823: Pmec-4-GFP(zdIs5) I;eat-3(tm1107) II
Genetic reagent
(C. elegans)
CZ16134: Pmec-4-GFP(zdIs5) I;npr-20(ok2575) II
Genetic reagent
(C. elegans)
CZ23845: Pmec-4-GFP(zdIs5) I;
ptps-1(tm1984) I
Genetic reagent
(C. elegans)
CZ17995: supr-1(ju1118) I;Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ12031: Pmec-4-GFP(zdIs5) I;rsef-1(ok1356) X
Genetic reagent
(C. elegans)
CZ17629: Pmec-4-GFP(zdIs5) I;
osm-7(tm2256) III
Genetic reagent
(C. elegans)
CZ17098: Pmec-4-GFP(zdIs5) I;
par-2(or373ts) III
Genetic reagent
(C. elegans)
CZ18676: Pmec-4-GFP(zdIs5) I;
rgl-1(ok1921) X
Genetic reagent
(C. elegans)
CZ20056: Pmec-7-GFP(muIs32) II;
wdfy-3(ok912) IV
Genetic reagent
(C. elegans)
CZ19721: Pmec-4-GFP(zdIs5) I
wdr-23(tm1817) I
Genetic reagent
(C. elegans)
CZ22063: Pmec-4-GFP(zdIs5) I;
brap-2(tm5132) II
Genetic reagent
(C. elegans)
CZ21217: Pmec-4-GFP(zdIs5) I;
brap-2(ok1492) II
Genetic reagent
(C. elegans)
CZ19337: Pmec-4-GFP(zdIs5) I;
cdc-48.1(tm544) II
Genetic reagent
(C. elegans)
CZ19725: Pmec-4-GFP(zdIs5) I;
ced-9(n1950sd) III
Genetic reagent
(C. elegans)
CZ21651: Pmec-4-GFP(zdIs5) I;
dnj-23(tm7102) II
Genetic reagent
(C. elegans)
CZ21356: Pmec-4-GFP(zdIs5) I;
fbxc-50(tm5154) II
Genetic reagent
(C. elegans)
CZ16950: Pmec-7-GFP(muIs32) II;
math-33(ok2974) V
Genetic reagent
(C. elegans)
CZ16951: Pmec-7-GFP(muIs32) II;
skr-5(ok3068) V
Genetic reagent
(C. elegans)
CZ21010: tep-1(tm3720) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ22796: mec-8(e398) I;Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ14510: Pmec-4-GFP(zdIs5) I;rict-1(mg360) II
Genetic reagent
(C. elegans)
CZ22570: rtcb-1(gk451) I /
[bli-4(e937) let-?(q782) qIs48](hT2) I, III;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ21655: Pmec-4-GFP(zdIs5) I;
skn-1(ok2315) IV/nT1(qIs51) IV; V
Genetic reagent
(C. elegans)
CZ23377: Pmec-4-GFP(zdIs5) I;
smg-3(r930) IV
Genetic reagent
(C. elegans)
CZ13997: Pmec-7-GFP(muIs32) II;
syd-9(ju49) X
Genetic reagent
(C. elegans)
CZ21723: Pmec-4-GFP(zdIs5) I;
tdp-1(ok803) II
Genetic reagent
(C. elegans)
CZ23133: Pmec-4-GFP(zdIs5) I;
wdr-5.1(ok1417) III
Genetic reagent
(C. elegans)
CZ21194: Pmec-7-GFP(muIs32) II;
mig-17(k174) V
Genetic reagent
(C. elegans)
CZ22792: Pmec-7-GFP(muIs32) II;
ZC116.3(ok1618) V
Genetic reagent
(C. elegans)
CZ19193: Pmec-7-GFP(muIs32) II;lgc-12(ok3546) III
Genetic reagent
(C. elegans)
CZ18217: Pmec-4-GFP(zdIs5) I;tmc-1(ok1859) X
Genetic reagent
(C. elegans)
CZ17639: Pmec-4-GFP(zdIs5) I;nud-1(ok552) III
Genetic reagent
(C. elegans)
CZ17841: Pmec-4-GFP(zdIs5) I;mgl-1(tm1811) X
Genetic reagent
(C. elegans)
CZ17843: Pmec-4-GFP(zdIs5) I;mgl-3(tm1766) IV
Genetic reagent
(C. elegans)
CZ17848: Pmec-4-GFP(zdIs5) I;npr-25(ok2008) V
Genetic reagent
(C. elegans)
CZ22890: Pmec-4-GFP(zdIs5) I;ucr-2.3(ok3073) III
Genetic reagent
(C. elegans)
CZ15607: drag-1(tm3773) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ17393: Pmec-4-GFP(zdIs5) I;
snb-6(tm5195) II
Genetic reagent
(C. elegans)
CZ17018: Pmec-4-GFP(zdIs5) I;
drag-1(tm3773) I
Genetic reagent
(C. elegans)
CZ18617: Pmec-4-GFP(zdIs5) I;
ect-2(ku427) II
Genetic reagent
(C. elegans)
CZ18818: Pmec-4-GFP(zdIs5) I;
lin-2(e1309) X
Genetic reagent
(C. elegans)
CZ18817: Pmec-4-GFP(zdIs5) I;
magi-1(zh66) IV
Genetic reagent
(C. elegans)
CZ20673: Pmec-4-GFP(zdIs5) I;
prmt-5(gk357) III
Genetic reagent
(C. elegans)
CZ18816: Pmec-4-GFP(zdIs5I;
rap-1(tm861) IV
Genetic reagent
(C. elegans)
CZ18460: Pmec-4-GFP(zdIs5) I;
smz-1(ok3576) IV
Genetic reagent
(C. elegans)
CZ22544: Pmec-4-GFP(zdIs5) I;
trxr-1(sv47) IV
Genetic reagent
(C. elegans)
CZ24963: Pmec-4-GFP(zdIs5) I;
natb-1(ju1405) V/nT1 IV; V
Genetic reagent
(C. elegans)
CZ16946: Pmec-7-GFP(muIs32) II;
rnf-5(tm794) III
Genetic reagent
(C. elegans)
CZ23068: ulp-5/tofu-3(tm3063) I;
Pmec-7-GFP(muIs32) II
Genetic reagent
(C. elegans)
CZ23091: Pmec-7-GFP(muIs32) II;
csr-1(fj54) IV/nT1 IV; V
Genetic reagent
(C. elegans)
CZ17638: Pmec-4-GFP(zdIs5) I;
elpc-3(ok2452) V
Genetic reagent
(C. elegans)
CZ12938: Pmec-4-GFP(zdIs5) I;
hda-6(tm3436) IV
Recombinant DNA reagent Plasmid: pCZGY3260:nmat-2 genomic DNA This work N/A nmat-2 genomic DNA (~1500 bp upstream;~670 bp downstream); modified pCFJ201
plasmid for modified MosSCI on ChIV
Recombinant DNA reagent Plasmid: pCZ993:Pmec-4-nmat-2 gDNA-let-858 3'UTR This work N/A nmat-2 expression driven by mec-4
promoter in the
mechanosensory neurons
Recombinant DNA reagent Plasmid: pCZ994: Pmtl-2-nmat-2 gDNA-let-858 3'UTR This work N/A nmat-2 expression driven by mtl-2 promoter in the intestine
Recombinant DNA reagent Plasmid: pCZ995: Pcol-12-nmat-2 gDNA-let-858 3'UTR This work N/A nmat-2 expression driven by col-12
promoter in the epiderdims
Recombinant DNA reagent Plasmid: pCZGY3329:
Pmec-4-GFP-ESYT-2
This work N/A
Recombinant DNA reagent Plasmid: pCZGY3344: Pmec-4-mKate2-ESYT-2 This work N/A
Recombinant DNA reagent Plasmid: pCZGY3342:
Pmec-4-mKate2-PISY-1
This work N/A
Recombinant DNA reagent Plasmid: pCZGY3302:
ivns-1 genomic DNA
This work N/A ivns-1 genomic DNA (2 kb upstream; 800 bp downstream)
Recombinant
DNA reagent
Plasmid: pCZGY3347: Prgef-1-mfsd-6 This work N/A
Recombinant
DNA reagent
Plasmid: pCZGY3346:Pesyt-2-GFP This work N/A GFP expression driven by esyt-2 promoter
Sequence-
based reagent
crRNA: nmat-2:/AltR1/rCrGrArGrU rCrGrC rUrCrU rUrCrU rUrGrC rCrGrUrGrUrU rUrUrA rGrArG rCrUrA rUrGrC rU/AltR2/ IDT N/A crRNA to makenmat-2(ju1512) and nmat-2(ju1514)
Sequence-
based reagent
crRNA: nmat-2:/AltR1/rCrGrUrGrU rUrGrA rArCrU rArArC rUrCrC rArCrU rGrUrU rUrUrA rGrArG rCrUrArUrGrC rU/AltR2/ IDT N/A crRNA to makenmat-2(ju1512)
Sequence-
based reagent
crRNA: nmat-1:/AltR1/rArA
rCrUrU rUrUrU rCrGrGrUrCrC rCrCrA rUrArG rGrUrU rUrUrA rGrArG rCrUrA rUrGrC rU/AltR2/
IDT N/A crRNA to mak
e nmat-1(ju1565)
Sequence-
based reagent
crRNA: nmat-1:/AltR1/rArU
rGrUrA rCrUrU rGrArU rUrArC
rGrGrA rArUrC rGrUrU
rUrUrA rGrArG rCrUrA
rUrGrC rU/AltR2/
IDT N/A crRNA to make nmat-1(ju1565)
Sequence-
based reagent
crRNA: qns-1:/AltR1/rGrGrUrGrU rUrArU rUrCrA
rCrGrU rGrUrU rArCrA
rGrUrU rUrUrA rGrArG
rCrUrA rUrGrC rU/AltR2/
IDT N/A crRNA to make qns-1(ju1563)
Sequence-based reagent crRNA: qns-1:/AltR1/rGrA
rUrArA rCrUrG rArArA rUrCrU
rGrGrA rUrArG rGrUrU rUrUrA
rGrArG rCrUrA rUrGrC rU/AltR2/
IDT N/A crRNA to make qns-1(ju1563)
Sequence-based reagent crRNA: esyt-2:/AltR1/rGrG rUrUrU rCrArG rUrArA rUrUrGrUrGrG rGrCrU rGrUrUrUrUrA rGrArG rCrUrA rUrGrC rU/AltR2/ IDT N/A crRNA to make esyt-2(ju1409)
Sequence-based reagent crRNA: esyt-2:/AltR1/rGrU rGrCrA rCrUrU rArCrG rGrGrU rUrGrU rArGrGrGrUrU rUrUrA rGrArGrCrUrA rUrGrC rU/AltR2/ IDT N/A crRNA to makeesyt-2(ju1409)
Peptide,
recombinant
protein
Protein: Cas9-NLS
purified protein
QB3 MacroLab, UC Berkley N/A
Peptide,
recombinant
protein
Phusion High-Fidelity DNA polymerases Thermo Scientific Cat#F530L
Peptide,
recombinant
protein
DreamTaq DNA polymerases Thermo Scientific Cat#EP0705
Commercial assay or kit
Chemical compound, drug 5-fluoro-2-deoxy uridine Sigma-Aldrich Cat#50-91-9
Software, algorithm ImageJ NIH image RRID:SCR_003070
Software, algorithm ZEN Zeiss https://www.zeiss.com/microscopy/us/downloads/zen.html
Software, algorithm Zeiss LSM Data Server Zeiss https://www.zeiss.com/microscopy/us/downloads/lsm-5-series.html
Software, algorithm GraphPad Prism 5 GraphPad
Software, Inc.
RRID:SCR_002798