Table 2. Mutants displaying increased PLM regrowth.
| Gene name | Mutations | Normalized regrowth (24 hr) | N | P value | Molecular function | Closest human Genea |
|---|---|---|---|---|---|---|
| A. Cell adhesion and ECM | ||||||
| emb-9 | tk75 b | 1.37 | 28 | *** | Collagen type IV α3 chain | COL4A3 |
| epi-1 | gm121 | 1.33 | 36 | *** | Laminin | LAMA |
| mig-17 | k174 | 1.24 | 37 | *** | ADAM metalloprotease | ADAMTS5 |
| ZC116.3 | ok1618 | 1.40 | 26 | *** | Cubilin | CUBN |
| B. Channels and transporters | ||||||
| lgc-12 | ok3546 | 1.33 | 26 | ** | Serotonin receptor 3E | HTR3E |
| tmc-1 | ok1859 | 1.31 | 30 | ** | Transmembrane channel-like protein | TMC1 |
| C. Cytoskeleton and motors | ||||||
| ivns-1 | ok3171 | 1.31 | 18 | ** | Actin-binding; splicing | IVNS1ABP |
| twf-2 | ok3564 | 1.33 | 39 | ** | Twinfilin actin binding protein | TWF |
| nud-1 | ok552 | 1.30 | 25 | ** | Nuclear distribution C, Dynein complex regulator | NUDC |
| tba-7 | gk787939 | 1.45 | 15 | *** | α-tubulin | TUBA |
| E. Neurotransmission, metabolism, and lipid | ||||||
| nmat-2 | tm2905 | 1.55 | 38 | *** | Nicotinamide mononucleotide adenylyltransferase | NMNAT1 |
| qns-1 | ju1563 | 1.13 | 40 | * | NAD + synthetase | NADSYN1 |
| mgl-1 | tm1811 | 1.24 | 28 | ** | Glutamate metabotropic receptor | GRM3 |
| mgl-3 | tm1766 | 1.32 | 22 | ** | Glutamate metabotropic receptor | GRM6 |
| npr-25 | ok2008 | 1.27 | 26 | ** | Coagulation factor II thrombin receptor | F2RL2 |
| ucr-2.3 | ok3073 | 1.41 | 24 | *** | Ubiquinol-cytochrome C reductase core protein | UQCRC2 |
| F. Trafficking | ||||||
| nex-1 c | gk148 | 1.38 | 27 | *** | Annexin | ANXA13 |
| nex-2 d | ok764 | 1.23 | 30 | ** | Annexin | ANXA7 |
| snb-6 | tm5195 | 1.30 | 38 | ** | Vesicle associated membrane protein | VAMP1 |
| G. Signaling and interactions | ||||||
| drag-1 | tm3773 | 1.52 | 26 | ** | Repulsive guidance molecule BMP co-receptor | RGMB |
| ect-2 | ku427 | 1.31 | 31 | ** | RhoGEF | ECT2 |
| lin-2 | e1309 | 1.31 | 27 | *** | Membrane associated guanylate kinase | CASK |
| magi-1 | zh66 | 1.40 | 29 | *** | Membrane associated guanylate kinase | MAGI2 |
| prmt-5 | gk357 | 1.26 | 24 | ** | Protein arginine N-methyltransferase | PRMT5 |
| rap-1 | tm861 | 1.33 | 10 | *** | Ras small GTPase | RAP1 |
| smz-1 | ok3576 | 1.39 | 13 | * | PDZ domain-containing protein | N/A |
| trxr-1 | tm2047 | 1.3 | 31 | * | Thioredoxin reductase | TXNRD2 |
| H. Protein turnover, proteases, cell death | ||||||
| natb-1 | ju1405 | 1.29 | 14 | * | N(α)-acetyltransferase 20 | NAA20 |
| rnf-5 | tm794 | 1.28 | 15 | * | Ring finger protein | RNF5 |
| ulp-5 | tm3063 | 1.22 | 30 | *** | SUMO specific peptidase | SENP7 |
| I. Gene expression and RNA regulation | ||||||
| csr-1 | fj54 | 1.24 | 38 | *** | Argonaute | AGO1 |
| hda-6 | tm3436 | 1.29 | 42 | *** | Histone deacetylase | HDAC6 |
| elpc-3 | ok2452 | 1.31 | 26 | *** | Elongator acetyltransferase complex subunit | ELP3 |
Genes are classified in nine functional or structural classes. Mutations are genetic or predicted molecular nulls, or partial loss-of-function. Normalized regrowth is relative to matched same-day controls or to pooled controls. Significant levels (*p<0.05; **p<0.01; ***p<0.001) based on Student’s t-test.
a Closest human gene based on BLASTP score in Wormbase WS263; Ensembl/HGNC symbol.
b emb-9(tk75) mutant reported to be a gain-of-function allele that makes stable EMB-9/Type IV collagen (Kubota et al., 2012).
cnex-1(gk148) mutant reported to show reduced regrowth in the C. elegans motor neurons (Nix et al., 2014).
d nex-2(bas4) mutant reported to show normal regrowth in the C. elegans motor neurons (Nix et al., 2014).