Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) |
Pkd2fl/fl | John Hopkins PKD Core |
PMID:20862291 | Mice with Pkd2 gene flanked by loxP regions |
| Strain, strain background (M. musculus) |
SMMHC-CreERT2Myh11-cre/ERT2 | Jackson Laboratories |
Stock # 019079 PMID:18084302 |
Mice with tamoxifen-inducible Cre recombinase that is expressed under the smooth muscle myosin heavy polipeptide 11 (Myh11) promoter. |
| Strain, strain background (M. musculus) |
Pkd2fl/fl:smCre+ | This paper | Mouse line created in-house by mating Pkd2fl/fl with SMMHC-CreERT2. Mice with inducible smooth muscle-specific deletion of PKD2. |
|
| Antibody | anti PKD2 (rabbit polyclonal) |
Baltimore PKD Core | Rabbit mAB 3374 CT-14/4 |
IF 1:200 dilution |
| Antibody | anti PKD2 (mouse monoclonal) |
Santa Cruz | Cat# sc-47734 RRID:AB_672380 |
WB 1:100 dilution, IF 1:100 dilution |
| Antibody | anti PKD2 (mouse monoclonal) |
Santa Cruz | Cat# sc-28331 RRID:AB_672377 |
WB 1:100 dilution, IF 1:100 dilution |
| Antibody | anti PKD1 (mouse monoclonal) |
Santa Cruz | Cat# sc-130554 | WB 1:100 dilution |
| Antibody | anti CaV1.2 (mouse monoclonal) |
UC Davis/NIH NeuroMab |
Cat# 73–053 RRID:AB_10672290 |
WB 1:100 dilution |
| Antibody | anti ANO1 (rabbit monoclonal) |
Abcam | Cat# ab64085 | WB 1:100 dilution |
| Antibody | anti TRPC6 (rabbit polyclonal) |
Abcam | Cat# ab62461 | WB 1:1000 dilution |
| Antibody | anti TRPM4 (rabbit polyclonal) |
Abcam | Cat# ab106200 | WB 1:500 dilution |
| Antibody | anti Actin (mouse monoclonal) |
Millipore Sigma | Cat# MAB1501 | WB 1:5000 dilution |
| Antibody | Alexa 555 secondary antibodies (anti rabbit and anti mouse) |
Thermofisher | Cat# A-21429 and # A-31570 |
IF 1:400 dilution |
| Other | Nuclear staining (DAPI) | Thermofisher | Cat# 3571 | IF 1:1000 dilution |
| Commercial assay or kit |
EZ-Link Sulfo-NHS -LC-LC-Biotin |
Thermofisher | Cat# 21338 | |
| Commercial assay or kit |
EZ-Link Maleimide-PEG2-Biotin |
Thermofisher | Cat# 21901BID | |
| Commercial assay or kit |
Mouse Angiotensin II ELISA kit |
Elabscience | Cat# E-EL-M2612 | |
| Commercial assay or kit |
Mouse Atrial Natriuretic Peptide ELISA kit |
Elabscience | Cat# E-EL-M0166 | |
| Commercial assay or kit |
Mouse Aldosterone ELISA kit |
Mybiosource | Cat# MBS775626 | |
| Chemical compound, drug |
Angiotensin II | Sigma-Aldrich | Cat# A9525 |