. 2016 Oct 4;215(10):1580–1589. doi: 10.1093/infdis/jiw470

Table 2.

Summary of Distinct CD8⁺ T-Cell Epitopes Recognized After Infection With Coxiella burnetii and Immunization With a Live, Recombinant, Attenuated Listeria monocytogenes Vaccine Expressing These Epitopes (Lm-Cb)

Identified Peptide ID	Function	Translocation Efficiency in Legionella,%^a	MHC Allele	Sequence	Stimulation Index^b
Identified Peptide ID	Function	Translocation Efficiency in Legionella,%^a	MHC Allele	Sequence	After C. burnetii Infection^c	After Lm-Cb Receipt^d
CBU2007_200–208	T4SS substrates	90	H-2-Db	FCYQNHTYF	5.11 ± 0.11	5.32 ± 0.28
CBU2052_83–91	T4SS substrates	90	H-2-Db	RASKNLLNY	4.99 ± 0.24	7.99 ± 0.52
CBU1569_173–181	T4SS substrates	90	H-2-Db	FQISNPPYL	4.78 ± 0.13	6.93 ± 1.46
CBU1198_27–35	T4SS substrates	75	H-2-Kb	SVVNYATLF	4.64 ± 0.29	2.34 ± 0.29
CBU1751_360–368	T4SS substrates	50	H-2-Db	VAIKSYRKF	3.96 ± 0.15	7.59 ± 0.46
CBU2007_292–300	T4SS substrates	90	H-2-Kb	FVPKFFLTF	3.73 ± 0.44	2.8 ± 0.52
CBU0414_137–145	T4SS substrates	45	H-2-Db	AQILNGDKL	3.45 ± 0.46	1.28 ± 0.57
CBU1823_136–144	T4SS substrates	50	H-2-Db	SGICSVATY	3.34 ± 0.04	4.59 ± 0.21
CBU0794_327–335	T4SS substrates	80	H-2-Db	SVNSNSGSF	3.24 ± 0.2	4.84 ± 0.71
CBU2052_236–244	T4SS substrates	90	H-2-Kb	TVLLRTPLF	3.13 ± 0.48	1.83 ± 0.32
CBU1460_114–122	T4SS substrates	40	H-2-Db	NALLDEETI	3.11 ± 0.39	6.63 ± 0.48
CBU0881_129–137	T4SS substrates	80	H-2-Kb	VALFILTHL	2.76 ± 0.44	1.94 ± 0.35
CBU0425_320–328	T4SS substrates	90	H-2-Kb	RVKVFLTHF	2.72 ± 0.35	2.44 ± 0.04
CBU0410_26–34	T4SS substrates	45	H-2-Db	NYATHKASY	2.44 ± 0.13	2.48 ± 0.10
CBU0425_73–81	T4SS substrates	90	H-2-Kb	ISLLRTLRY	2.25 ± 0.06	1.91 ± 0.35
CBU1626(IcmG/DotF)_37–45	T4SS component	…	H-2-Kb	KPANFFVRF	4.44 ± 0.2	2.47 ± 0.18
CBU1628(IcmK/DotH)_20–28	T4SS component	…	H-2-Kb	ASIIPVLAL	3.93 ± 0.26	2.39 ± 0.18
CBU1648(DotA)_5–13	T4SS component	…	H-2-Db	SSLLASISL	3.83 ± 0.22	7.61 ± 0.04
CBU1648(DotA)_668–676	T4SS component	…	H-2-Kb	IVVYTFSGL	3.48 ± 0.2	2.87 ± 0.04
CBU1626(IcmG/DotF)_52–60	T4SS component	…	H-2-Kb	ITAVIAVVL	2.97 ± 0.18	1.94 ± 0.09
CBU1628(IcmK/DotH)_106–114	T4SS component	…	H-2-Db	NTIRNMMPL	2.94 ± 0.33	4.24 ± 0.10
CBU1628(IcmK/DotH)_165–173	T4SS component	…	H-2-Kb	SGFVTSLVF	2.32 ± 0.26	2.42 ± 0.23
CBU_0311_119–127	Immunodominant	…	H-2-Kb	SNYSYRTRL	4 ± 0.57	8.37 ± 0.67
CBU_0092_72–80	Immunodominant	…	H-2-Kb	TVTHRLARL	3.74 ± 0.57	8.28 ± 0.16
CBU_0092_194–202	Immunodominant	…	H-2-Kb	AQASFQNYL	3.54 ± 0.15	9.04 ± 0.23
CBU_0311_186–194	Immunodominant	…	H-2-Kb	RHYFMNNVF	3.49 ± 0.37	7.95 ± 0
CBU1910(Com1)_213–221	Immunodominant	…	H-2-Db	LQLAGTPTF	3.45 ± 0.31	6.53 ± 0.32
CBU_1718_181–189	Immunodominant	…	H-2-Db	SGLENALEV	2.84 ± 0.22	1.92 ± 0.40
CBU_0388_770–778	Immunodominant	…	H-2-Db	YSAQNEISF	2.76 ± 0.15	2.34 ± 0.15

Abbreviations: ID, identifier; MHC, major histocompatibility complex; SD, standard deviation.

^a The translocation efficiency of C. burnetii T4SS substrates by Legionella pneumophila is reported by Weber et al [21].

^b The stimulation index was determined by an interferon γ enzyme-linked immunospot assay and was calculated using the number of spot-forming cells (SFCs) in peptide-stimulated cells divided by the number of SFCs in medium-stimulated cells. A stimulation index of > 2 was considered to be positive.

^c A total of 2 × 10⁵ CD8⁺ T cells were isolated on day 10 after infection from 5 mice infected with 1 × 10⁶C. burnetii. Each peptide was added at a concentration of 2 µg/mL for 20 hours. The data presented are the mean (±SD) of 3 independent experiments.

^d A total of 2 × 10⁵ CD8⁺ T cells were isolated on day 10 after infection from 5 mice immunized with 5 × 10⁶ Lm-Cb. Each peptide was added at a concentration of 2 µg/mL for 20 hours. The data presented are the mean (±SD) of 3 independent experiments.