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. 2009 Aug 26;64(5):938–944. doi: 10.1093/jac/dkp315

Table 1.

Primers used in the OLAs for discriminating between variants tested in this study

PR position Upstream primer OLA target Downstream primer
74 5′-CGGAUAUAAAGCTATAGGTA-3′ 74T 5′-CAGTAUTAGTAGGACCTACA-3′
5′-CGGAUAUAAAGCTATAGGTT-3′ 74S
15 5′-ACCCCTCGTCACAATAAAGA-3′ subtype B (15I) 5′-TAGGGGGGCAATTAAAGGAA-3′
5′-ACCCCTCGTCACAATAAAGG-3′ subtype C (15V)
54 5′-AGGGGGAATTGGAGGTTTTA-3′ wild-type (54I) 5′-TCAAAGTAAGACAGTATGAT-3′
5′-AGGGGGAATTGGAGGTTTTG-3′ multiresistant (54V)