Figure 2.

Pyrosequencing results. Pyrograms from a positive control, chloroquine-resistant strain Dd2 (A); a negative control, chloroquine-sensitive strain HB3 (B); and a field sample from Mwanza district with evidence of mixed chloroquine-resistant and chloroquine-sensitive infections (C). Note presence of guanine in the 76th codon of the field sample, which is the second nucleotide added in pyrosequencing, indicating the presence of chloroquine-resistant parasites. This field sample also contains adenosine in the 75th codon, which is the fourth nucleotide added, indicating the presence of chloroquine-sensitive parasites.