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. 2016 Mar 22;25(11):2168–2181. doi: 10.1093/hmg/ddw084

Figure 1.

Figure 1.

miRNA expression changes in spinal motor neurons after acute SMN knockdown. ( A ) Schematic of experimental paradigm for SMN knockdown experiments. shRNA-mediated knockdown significantly decreases SMN mRNA (in B ) and protein (in C ) after 6 days of infection with shSMN or shCtl, normalized to Gapdh expression. N = 8 from four biological replicates, *** P < 0.001, unpaired two-tailed Student’s t -test. ( D ) LNA-based miRNA microarray identified 56 differentially expressed miRNAs ranked by hierarchical clustering following loss of SMN expression. ( E ) Table of top differentially expressed miRNA with P -value <0.02 after multiple corrections and > 1.25-fold change. ( F ) qRT-PCR validation of expression changes of the top 10 miRNAs identified by microarray normalized to miR-191 and to shCtl expression. N = 6 from four different experiments. * P < 0.05, *** P < 0.001 by unpaired two-tailed Student’s t -test. Data are represented as mean ± SEM.