Skip to main content
. 2016 Mar 22;25(11):2168–2181. doi: 10.1093/hmg/ddw084

Figure 6.

Figure 6.

Inhibition of miR-431 expression after acute SMN knockdown ameliorates the motor neuron neurite outgrowth phenotype. ( A ) Two representative images of Tau staining neurites in low density cultured wild-type spinal motor neurons transfected with 30 nM mimic-Ctl or mimic-431 for 72 h and fixed at 4DIV. Neurite tracing of ( B ) the total neurite and ( C ) longest Tau-stained neurite with a minimum cutoff of 3× the length of the cell body. N > 148 from 3 experiments. Mann–Whitney, **** P < 0.0001. ( D ) Antimir inhibition of miR-431 in SMN knockdown motor neurons rescues both total neurite length and ( E ) the longest neurite length. N > 160 from 3 experiments. Kruskal–Wallis with Dunns Multiple Comparisons post-test, * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are represented as mean ± SEM.