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Measurement of cellular and mitochondrial ATP levels. Mutant and control cell lines were incubated with 10 mM glucose or 5 mM 2-deoxy-d-glucose plus 5 mM pyruvate to determine ATP generation under mitochondrial ATP synthesis. Average rates of ATP level per cell line in mitochondria are show. Six to seven determinations were made for each cell line. Graph details and symbols are explained in the legend to Figure 2.
