Figure 7.

Mitochondrial membrane potential analysis. The mitochondrial membrane potential (ΔΨ_m) was measured in mutant and control cell lines using a fluorescence probe JC-10 assay system. The ratio of fluorescence intensities Ex/Em = 490/590 nm and 490/530 nm (FL₅₉₀/FL₅₃₀) were recorded to delineate the ΔΨ_m level of each sample. The relative ratios of FL₅₉₀/FL₅₃₀ geometric mean between mutant and control cell lines were calculated to reflect the level of ΔΨ_m. Relative ratio of JC-10 fluorescence intensities at Ex/Em = 490/530 nm and 490/590 nm in absence (A) and presence (B) of 10µM of carbonyl cyanide 3-chlorophenylhydrazone (FCCP). The average of 3-5 determinations for each cell line is shown. Graph details and symbols are explained in the legend to Figure 2.