Fig. 1.

CCN2 promotes the cell proliferation of C2C12 cells. (A) C2C12 cells were stimulated for 16 h with rCCN2 at the indicated concentrations in differentiation medium. Then, cell proliferation assay was performed. Each bar shows the mean value and standard deviation of the results from nine wells. **P < 0.01 indicates significant difference from control by one-way ANOVA. (B) C2C12 cells were treated with rCCN2 at the indicated concentrations for 24 h. Western blot analysis was then performed by using anti-PCNA and anti-β-actin antibodies. (C) C2C12 cells were transiently transfected with CCN2 expression plasmid containing an HA tag, and the cells were cultured for 4 days. Western blot analysis was performed by using antibodies against the indicated antigens.