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. 2014 Sep 26;157(2):91–100. doi: 10.1093/jb/mvu056

Fig. 2.

Fig. 2

CCN2 regulates the gene expression and protein production of MyoD and myogenin in C2C12 cells. (A) C2C12 cells were stimulated with rCCN2 at the indicated concentrations in differentiation medium. After 1 and 2 days, the gene expression and protein production of MyoD were examined, respectively. *P < 0.05 indicates significant difference by one-way ANOVA. (B) C2C12 cells were treated with rCCN2 in differentiation medium. After 4 and 5 days, the gene expression and protein production of myogenin were evaluated, respectively. *P < 0.05 indicates significant difference by one-way ANOVA. (C) C2C12 cells were stimulated with rCCN2 in differentiation medium. At the indicated time after stimulation, cell lysate was collected and western blot analysis was performed.