Fig. 3.

CCN2 regulates the gene expression of Desmin (A), MHC (B), and Myostatin (C) in C2C12 cells. C2C12 cells were stimulated with rCCN2 at the concentrations indicated for 4 days, and real-time PCR analysis was performed. The amounts of these gene products were normalized to that of Gapdh with respect to the control sample (ratio = 1.0). Bars represent means and standard deviations of results obtained from eight to nine different cultures for each sample. *P < 0.05 and **P < 0.01 indicate significant differences from control by one-way ANOVA.