Fig. 4.

CCN2 production is increased in C2C12 cells treated with TNFα. (A) C2C12 cells were stimulated by 20 ng/ml TNFα in differentiation medium. At the indicated time the cell lysates were collected and western blot analysis was performed by using anti-CCN2 and β-actin antibodies. (B) C2C12 cells were treated with TNFα at the indicated concentrations for 2 days. Then, western blot analysis was similarly performed. (C) Quantification of the results obtained from the experiments for panel A at 48 h in triplicates. *P < 0.05 indicates significant difference from control by Student’s t-test.