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. Author manuscript; available in PMC: 2018 Dec 5.
Published in final edited form as: Pharmacogenet Genomics. 2011 Sep;21(9):539–551. doi: 10.1097/FPC.0b013e328348c76b

Figure 2. UPLC and MS/MS analysis of OLZ glucuronides formed by HLM.

Figure 2.

Glucuronidation assays were performed using 12.5 μg HLM protein and 300 μM OLZ, and incubated at 37°C for 2 h with 4 mM UDP-glucuronic acid prior to analysis by UPLC as described in the Materials and Methods. Panel A, HLM + vehicle (1% DMSO); panel B, UGT-null HEK 293 cell homogenates + OLZ; panel C, HLM + OLZ; panel D, HLM + OLZ and 1,000 U of β-glucuronidase . Panel E, mass spectra of UPLC peaks 1 and 2; panel F, mass spectra of peak 3; Peak 1, OLZ-10-N-glucuronide isomer 1; peak 2, OLZ-10-N-glucuronide isomer 2; peak 3, OLZ-4’-N-glucuronide; peak 4, OLZ; peak 5, UDP-glucuronic acid. AU, absorbance.