Table 1. Real-time PCR primers used for gene expression analysis.
| Gene | Forward | Reverse | Amplicon (bp) |
|---|---|---|---|
| GAPDH | GGCAAATTCAACGGCACAGT | GTCTCGCTCCTGGAAGATGG | 84 |
| HPSE | CAAGAACAGCACCTACTCAAG | AGCAGTAGTCAAGGAGAAGC | 155 |
| αSMA | TGCTGGACTCTGGAGATGGT | ACGAAGGAATAGCCACGCTC | 148 |
| VIM | TCCAGAGAGAGGAAGCCGAA | AAGGTCAAGACGTGCCAGAG | 75 |
| COLL1 | GAGTGGAAGTGTGAGCGACA | GGTGAGTCTGCGGTTGGTAA | 97 |
| TGFβ | GTGTGGAGCAACATGTGGAACTCTA | CGCTGAATCGAAAGCCCTGTA | 174 |
| IL6 | CTGCAAGAGACTTCCATCCAGTT | GAAGTAGGGAAGGCCGTGG | 70 |
| IL1β | TGTTTTCCTCCTTGCCTCT | TGCCTAATGTCCCCTTGA | 100 |
| TNFα | CATCTTCTCAAAATTCGAGTGACAA | TGGGAGTAGACAAGGTACAACCC | 175 |
| FN | QuantiTec Primer Assay (Quiagen) | Cat. No. QT00135758 | |
| MMP9 | CTTGAAGTCTCAGAAGGTGGATC | CGCCAGAAGTATTTGTCATGG | 135 |
| MMP2 | TGCCATCCCTGATAACCTGGAT | CTCTTCAGACTTTGGTTCTCC | 108 |
| ET-1 | TGCTGTTCGTGACTTTCC | TGTTGACCCAGATGATGTC | 198 |
| EDNRA | GCTGGTTCCCTCTTCACTTAAGC | TCATGGTTGCCAGGTTAATGC | 129 |
| eNOS | CTGGCAGCCCCAAGACCTA | GTGACATCGCCGCAGACAA | 112 |
| iNOS | CAGCTGGGCTGTACAAACCTT | CATTGGAAGTGAAGCGTTTCG | 95 |