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. 2018 Nov 16;9(90):36166–36184. doi: 10.18632/oncotarget.26322

Figure 1. Treg cells populate human mucosal sites.

Figure 1

(A) Treg and Tconv cells from blood (upper panel), lung (middle panel) and colon (lower panel) were flow cytometry isolated, rested overnight and treated with PMA/Ionomycin for 6h in the presence of BD GolgiStop and BD GolgiPlug. Expression of IL-2 and FOXP3 was analyzed by flow cytometry. The inset numbers represent the percentage of the gated cells in the respective gating step. Dot plots are representative examples of four independent experiments. (B) Mean frequency (± SD) of IL-2+FOXP3– and IL-2−FOXP3+ Tconv and Treg cells. Individual symbols represent data from individual tissue specimens. Small horizontal lines indicate the mean. (C) CD69 and CD103 expression on Treg and Tconv cells shown as representative flow cytometry plots. (D) Graph shows compiled flow cytometry data for CD69 and CD103 expression (n = 7–11). Individual symbols represent data from individual tissue specimens. Small horizontal lines indicate the mean (±SD).