Table 1. ATPase activity of Rlip and its mutants*.
| Protein | specific activity (nmol/min/mg) | |
|---|---|---|
| DOX | DNP-SG | |
| RLIP76 | 275 ± 7 | 220 ± 15 | 
| M1 | 145 ± 1 | 95 ± 11 | 
| M2 | 105 ± 20 | 75 ± 4 | 
| M3 | 150 ± 1 | 175 ± 4 | 
| M4 | 205 ± 16 | 60 ± 7 | 
*Mutants designated as M1 through M4 were mutated at the following residues: M1 (Y231A;R232A;V233K,S234A); M2 (A264R;S265A;L267A;K268A); M3 (Q340A;N341A); M4 (I344A;V345A)