Table 1. Applied primer (A) and probe (B) sequences were used to analyze the PCR bias and amplification efficiency, and identify rare amounts of methylated DNA at a high background of unmethylated wild-type DNA.

A
Target	Forward (5′->3′)	Reverse (5′->3′)	Amplicon length
PLA2R1	GGGGTAAGGAAGGTGGAGAT	ACAAACCACCTAAATTCTAATAAACAC	168 bp
PLA2R1	GGGGTAAGGAAGGTGGAGAT	ACCTAAATTCTAATAAACACCGC	161 bp
PLA2R1	GGGGTAAGGAAGGTGGAGAT	AATAAACACCGCGAATTTACAAC	150 bp
PLA2R1	GGAAGGTGGAGATTACGG	GCGAATTTACAACGAACAAC	133 bp
B
Target	methylated (5′->3′)		unmethylated (5′->3′)
PLA2R1	CCCAACTACTCCGCGACGCAA		AACCCAACTACTCCACAACACAAA