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. Author manuscript; available in PMC: 2019 Apr 22.
Published in final edited form as: Nat Methods. 2018 Oct 22;15(11):955–961. doi: 10.1038/s41592-018-0167-z

Figure 4. Classification of translating mammalian 80s ribosome with 3D-CTF compensated missing-wedge and multi-scale PCA.

Figure 4.

(a-c) Classes I-III represent a post translocational state with the co-factors shown in the lower row from the inter-subunit surface with the 60s subunit removed for clarity. (d-e) Classes IV-V have a mid-rotated 40s and a swiveled head corresponding to a pre-translocational intermediate. Scale bar represents 100 Å. (f-g) Class IV in dark blue overlaid with class III in gold, showing the mid-rotated 40s state. (h-i) MDFF of eeF2 (orange) with the density from class-IV (h) and class-V (i) starting from PDB-4ujo (cyan ribbon). (j-k) The rigid body docking of PDB-4ujo into the density from class IV/V, respectively. Arrows point to this density which is occupied by the antibiotic Sordarin in PDB-4ujo, but is not present in the sample used in this study.