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. Author manuscript; available in PMC: 2018 Dec 5.
Published in final edited form as: Chem Res Toxicol. 2015 Oct 28;28(11):2112–2119. doi: 10.1021/acs.chemrestox.5b00278

Figure 3. Concentration curves and kinetic analysis for (R)-NNAL- and (S)-NNAL-Gluc formation with microsomes from UGT2B7, UGT2B17, UGT1A9 and UGT2B10 overexpressing cells.

Figure 3.

Glucuronide formation assays were performed at 37 °C for 1 h using 15-20 μg total UGT-overexpressing cell microsomal protein as described in the Materials and Methods. Rate, Vmax and Vmax/KM values are expressed per mg of total protein. Representative curves are shown and kinetic analysis was performed in three independent experiments.