Fig. 3. METTL3 functions as an oncogene in NPC.

a METTL3 and METTL14 mRNA expression level in HNSC in the TCGA dataset. b The relative mRNA expression of METTL3 in normal nasopharyngeal epithelial cell NP69 and various NPC cell lines, as assessed by quantitative RT-PCR. c Disease-free survival (DFS) analysis in HNSC according to the METTL3 or METTL14 expression in the TCGA dataset. d The correlation of METTL3 and ZNF750 mRNA expression in HNSC, as assessed by Pearson analysis. e Pearson analysis of the correlation between ZNF750 and METTL3 mRNA expression in NPC cell lines. The blue dots represent ZNF750 and METTL3 mRNA expression in NP69, 5–8 F, 6–10B, C666–1, HNE1, HONE1, S18, S26, and SUNE1 cells. CT: cycle threshold. f Western blotting detection of METTL3 levels in NPC cells with shRNA knock down of METTL3. g, h Quantitative RT-PCR detection of ZNF750 expression in NPC cells with METTL3 ectopic expression or knockdown. *p < 0.05, **p < 0.01, ***p < 0.001