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. 2018 Dec 5;8:17651. doi: 10.1038/s41598-018-36121-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phospho flow measurements are highly reproducible. Basal STAT3 (pY705) signals were analyzed in normal B cells from four healthy blood donors (N1-N4) before (fresh) and after (cryo) cryopreservation, and in cryopreserved B cells from three CLL patients (CLL105, CLL135, CLL136). Symbols with a cross represent CLL patients with unmutated IgVH status. The analysis was done on three samples from each donor on three different days, following the procedure described in Methods.