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. 2018 Dec 5;8:17651. doi: 10.1038/s41598-018-36121-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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sCD40L induced signaling is not significantly affected by cryopreservation. B cells from the same two healthy donors were analyzed before (fresh) and after (cryo) cryopreservation. The cells were stimulated with sCD40L (400 ng/mL) for 5 and 10 min before fixation and phospho flow analysis as described in Methods. Results are shown as mean ± standard deviation. Statistical significance was calculated between the two groups (fresh, cryo) by an ordinary two-way ANOVA with Sidak’s multiple comparisons test. **p < 0.01, *p < 0.05.