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. 2018 Dec 5;8:17651. doi: 10.1038/s41598-018-36121-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Signaling responses induced by ibrutinib treatment is unaffected by cryopreservation. B cells were isolated from healthy blood donors and analyzed immediately (n = 3; fresh) or after cryopreservation (n = 3; cryo). The cells were treated with the indicated concentrations of ibrutinib for 20 min before stimulation with anti-IgM (10 μg/mL) for three min. The cells were then fixed and analyzed by phospho flow as described in Methods. Results are shown as mean ± standard deviation. Statistics were performed as in Fig. 4. No significant p-values were achieved.