Fig. 3. DANCR interacts with RXRA in TNBC cells.

a Schematic diagram of putative RXRA binding sites in DANCR. b RIP-qPCR assay of the association of RXRA with DANCR in MDA-MB-231 and MDA-MB-468 cells. c Re-expression of shRNA-resistant DANCR wild type and RXRA-binding mutant types. d RIP-qPCR assay of effects of re-expression of shRNA-resistant DANCR wild type or mutant types on RXRA binding. e Biotinylated DANCR was incubated with nuclear extracts (MDA-MB-231 and MDA-MB-468 cells), targeted with streptavidin beads, and binding proteins were resolved in a gel. Western blotting assay of the specific binding of RXRA and DANCR. f, g RNAs corresponding to fragments in different regions of DANCR were treated as in (e), and binding RXRA was detected by western blotting assay. Error bars ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. Data are representative from two independent experiments