Fig. 2. miR-4658 targets CCSlnc362 containing rs1317082 [C] allele in CRC.

a, b Predicted folding structures and minimum free energy (MFE) for CCSlnc362 with rs1317082 [T] or rs1317082 [C]. We used RegRNA 2.0 and RNAhybird. c Predicted interaction between miR-4658 and CCSlnc362. d, e Relative luciferase activities of CCSlnc362 fragment with the rs1317082 [T] or rs1317082 [C] cotransfected with miR-4658 mimics or miR-4658 inhibitor were calculated in HCT116 cells. f Relative expression of CCSlnc362 was detected in SW1116 (genotype: CC) and DLD-1 cells (genotype: TT) transfected with miR-4658 mimics. All the experiments were repeated for at least three times and four replicates were conducted for each group