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. 2018 Dec 5;4:111. doi: 10.1038/s41420-018-0131-9

Fig. 1. Annexin V and DNA fragmentation assays of human polymorphonuclear cells (PMNCs).

Fig. 1

a Freshly isolated human peripheral white blood cells were analyzed by flow cytometry for annexin V staining. MNCs and PMNCs were gated using forward scatter and side scatter, and annexin V staining was analyzed. b Annexin V and PI staining at 0, 3, and 12 h after blood collection in PMNCs. c May–Giemsa staining shows the separated MNCs and PMNCs. d DNA laddering analyzed immediately after separating MNCs and PMNCs. e DNA fragmentation of PMNCs and MNCs analyzed after ultraviolet irradiation for 15 min. “M” indicates the DNA marker. f TUNEL assay using separated MNCs and PMNCs; N = 6