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. 2018 Dec 5;4:111. doi: 10.1038/s41420-018-0131-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Freshly isolated human peripheral white blood cells were analyzed by flow cytometry for annexin V staining. MNCs and PMNCs were gated using forward scatter and side scatter, and annexin V staining was analyzed. b Annexin V and PI staining at 0, 3, and 12 h after blood collection in PMNCs. c May–Giemsa staining shows the separated MNCs and PMNCs. d DNA laddering analyzed immediately after separating MNCs and PMNCs. e DNA fragmentation of PMNCs and MNCs analyzed after ultraviolet irradiation for 15 min. “M” indicates the DNA marker. f TUNEL assay using separated MNCs and PMNCs; N = 6