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. 2018 Dec 5;4:111. doi: 10.1038/s41420-018-0131-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Immunohistochemical staining of MNCs and PMNCs for LC3. The percentage of LC3-positive cells in each fraction was determined by cell counting under a microscope. b Western blotting for LC3. c Inhibition of autophagy with 3-MA. Human mononuclear cells derived from the bone marrow were incubated with cytokines in the presence or absence of 3-MA at a final concentration of 10 nM. d Inhibition of apoptosis with the caspase inhibitor, zVAD-fmk (100 µM); N = 6