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. 2018 Dec 5;8:17654. doi: 10.1038/s41598-018-36182-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fbln7-FL and Fbln7-C inhibit 7KCh-induced neovascularization in vivo. (A) Surgical procedure for the rat corneal angiogenesis model. (B) Rat corneal angiogenesis model. Implants containing 300 ng protein/implant were placed into the anterior chamber, and fluorescent images were taken 10 days post-implantation. Three different implants were inserted: 7KCh and BSA (control), 7KCh and Fbln7-FL, and 7KCh and Fbln7-C. White dotted circles indicate implants. White triangles indicate neovasculature from limbus towards the implant. L: Limbus; triangles indicate neovasculature; scale bar: 1 mm. (C) Protein dose-dependent experiments in the rat corneal angiogenesis model. Neovessel area was calculated for each experiment, with 6, 60, and 300 ng protein in the implants. N = 6, *P < 0.05.