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. 2018 Dec 5;8:17654. doi: 10.1038/s41598-018-36182-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fbln7-C affects focal adhesion area and actin filaments to inhibit cell motility. (A–C) Cell motility on fibronectin or Fbln7-C-coated dishes stimulated with VEGF and analyzed using time-lapse imaging. Cell motility is evaluated by (A) cell velocity, (B) distance, (C) and persistence. N = 3 n > 150, ****P < 0.0001, **P < 0.01, *P < 0.01. (D–I) Cell morphology differences between cells on fibronectin and Fbln7-C-coated dishes evaluated by staining for focal adhesion sites (paxillin; red) and actin filaments (phalloidin; green). (D) Immunofluorescence staining. Cell shape is evaluated by (E) shape factor and (F) number of lamellipodia. Focal adhesion area is evaluated by (G) average focal adhesion area, (H) focal adhesion number and (I) cell area. FN: Fibronectin, F7C:Fibulin7-C, scale bar: 100 μm, N = 3 n > 80, ****P < 0.0001, **P < 0.01, *P < 0.01.