Fig. 5. MiR-627-5p mediated the tumor-suppressive effects of UCA1 knockdown on glioma cell lines.

a Cell Counting Kit-8 (CCK-8) assay was used to measure the effect of UCA1 and miR-627-5p on cell proliferation in U87 and U251 cells. b Transwell assay was used to evaluate the effect of UCA1 and miR-627-5p on cell migration and invasion of U87 and U251 cells. c Flow cytometry analysis was used to evaluate the effect of effect of UCA1 and miR-627-5p on cell apoptosis of U87 and U251 cells. Error bars represent as the mean ± SD (n = 3, each group). *P < 0.05, ^#P < 0.05. Scale bars represent 20 μm. d Western blot analysis for Nuclear receptor subfamily 2 group C member 2 (NR2C2) expression level in U87 and U251 cells. Error bars represent as the mean ± SD (n = 3, each group). *P < 0.05, ^##P < 0.01. Scalebars represent 20 μm