Fig. 6. Interplay of NR2C2 and miR-627-5p.

a Cell Counting Kit-8(CCK8) assay to evaluate the effect of miR-627-5p and NR2C2 on cell proliferation in U87 and U251 cells. b Transwell assay to evaluate the effect of miR-627-5p and NR2C2 on cell migration and invasion of U87 and U251 cells. c Flow cytometry analysis to evaluate the effect of miR-627-5p and NR2C2 on cell apoptosis of U87 and U251 cells. Error bars represent as the mean ± SD (n = 3, each group). *P < 0.05, ^#P < 0.05, ^&P < 0.05. Scale bars represent 20 μm. d Western blot analysis for SPARC/osteonectin, cwcv, and kazal-like domains proteoglycan 1 (SPOCK1) in U87 and U251 cells with the expression of miR-627-5p and NR2C2 changed. Error bars represent as the mean ± SD (n = 3, each group). **P < 0.01, ^##P < 0.01, ^&&P < 0.01, ^▲▲▲P < 0.001